Goat Anti-PRMT5 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09534
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
SKB1 homolog|HMT1 hnRNP methyltransferase-like 5|SKB1Hs|SKB1|JBP1|IBP72|HRMT1L5|protein arginine methyltransferase 5|PRMT5
Usage Summary
Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_006100.2; NP_001034708.1; NP_001269882.1; NP_001269883.1; NP_001269884.1; NP_001269885.1
Blocking Peptide
EBP09534
Immunogen
Peptide with sequence RACREKDRDPEAQ, from the internal region of the protein sequence according to NP_006100.2; NP_001034708.1; NP_001269882.1; NP_001269883.1; NP_001269884.1; NP_001269885.1.
Product Comments
This antibody is expected to recognize both isoforms (NP_006100.2; NP_001034708.1).
Peptide Sequence
RACREKDRDPEAQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human
Human Gene ID
10419
Mouse Gene ID
27374
Rat Gene ID
364382
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary testing showed a band at approx. 70kDa in A431 and HepG2 nuclear cell lysate after 1-2ug/ml antibody staining. (calculated MW of 72.7kDa according to NP_006100.2). Primary incubation 1 hour at room temperature
Application Type
Pep-ELISA, IF, FC

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