Goat Anti-Pro-EGF Antibody

$423.00

Category: Primary Antibodies
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SKU: EB11752
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
URG|pro-epidermal growth factor|OTTHUMP00000219722|OTTHUMP00000219721|OTTHUMP00000162922|HOMG4|epidermal growth factor|EGF|beta-urogastrone
Accession ID
NP_001954.2; NP_001171601.1; NP_001171602.1
Blocking Peptide
EBP11752
Immunogen
Peptide with sequence SRQERVCNIEKNVS, from the internal region (near the N terminus) of the protein sequence according to NP_001954.2; NP_001171601.1; NP_001171602.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_001954.2; NP_001171601.1; NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.
Peptide Sequence
SRQERVCNIEKNVS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog
Reactive Species
Human
Human Gene ID
1950
Product Grade
IHC Results
Paraffin embedded Human Skin. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 160kDa double band observed in Human Prostate lysates (calculated MW of 134kDa according to NP_001954.2). The two bands represent glycosylated forms of the mature protein. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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