Goat Anti-PTBP2 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

splicing regulator|neural polypyrimidine tract binding protein|PTB-like|nPTB8|nPTB7|nPTB6|nPTB5|nPTB|brPTB|PTBLP|PTB|MIBP|FLJ34897|polypyrimidine tract binding protein 2|PTBP2

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 cells. Recommended concentration: 10µg/ml.

NP_001287914.1; NP_001287915.1; NP_001287916.1; NP_001287917.1; NP_001287918.1; NP_067013.1 ;

EBP08108

Peptide with sequence C-SKKFKGEDKMD, from the internal region of the protein sequence according to NP_001287914.1; NP_001287915.1; NP_001287916.1; NP_001287917.1; NP_001287918.1; NP_067013.1 ;.

This antibody is expected to recognise NP_001287914.1 (isoform 1), NP_001287915.1 (isoform 2), NP_001287916.1 (isoform 3), NP_001287917.1 (isoform 4), NP_001287918.1 (isoform 5), and NP_067013.1 (isoform 6).

C-SKKFKGEDKMD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Cow

Human, Mouse

58155

56195

310820

Antibody detection limit dilution 1:1000.

Approx 55-60kDa band observed in Mouse Testis lysates and in nuclear lysates of cell line NIH3T3, and approx 55kDa in preliminary testing at Rat testes lysate (calculated MW of 57.6kDa according to Mouse NP_062423.1 and 57.5kDa according to NP_001297640.1 ). Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IF