Goat Anti-Radixin Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07306
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
radixin|RDX
Accession ID
NP_002897.1
Blocking Peptide
EBP07306
Immunogen
Peptide with sequence C-QARDETKKTQND, from the internal region of the protein sequence according to NP_002897.1.
Peptide Sequence
C-QARDETKKTQND
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Mouse, Rat
Human Gene ID
5962
Mouse Gene ID
19684
Rat Gene ID
315655
Product Grade
IHC Results
Paraffin embedded Human Placenta and Kidney. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1). The same 80kDa was also observed in lysates of several Mouse and Rat tissues and in lysates of cell lines NIH3T3, HeLa, Jurkat, and A431. The observed molecular weight corresponds to earlier findings in literature (Magendantz et al, J Biol Chem. 1995 Oct 27;270(43):25324-7. PMID: 7592691). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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