Goat Anti-RICTOR Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

TORC2-specific protein AVO3|mAVO3|PIA|RPTOR independent companion of MTOR, complex 2|RICTOR|DKFZp686B11164|pianissimo|mAVO3|rapamycin-insensitive companion of mTOR|likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae)|MGC39830|AVO3|KIAA1999|Rictor

NP_689969.2

EBP06732

Peptide with sequence C-KQPIVDTSAES, from the C Terminus of the protein sequence according to NP_689969.2.

C-KQPIVDTSAES

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Dog

Human

253260

78757

In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3µg/ml.

Antibody detection limit dilution 1:4000.

Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC