Goat Anti-RNF13 / RZF (isoform 1 and 3) Antibody

$464.00

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SKU: EB05854
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FLJ93817|MGC13689|RING zinc finger protein|RZF|ring finger protein 13|RNF13
Usage Summary
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_009213.1; NP_001365216.1; NP_001365220.1
Blocking Peptide
EBP05854
Immunogen
Peptide with sequence C-PNGERDYNIANTV, from the C Terminus of the protein sequence according to NP_009213.1; NP_001365216.1; NP_001365220.1.
Product Comments
Reported variants represent identical protein (NP_009213.1; NP_899237.1).
Peptide Sequence
C-PNGERDYNIANTV
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
11342
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:1000.
Western Blot
Approx. 42kDa band observed in lysates of cell line A431 and in preliminary testing of Human Lung lysate, and approx. 45kDa in lysates of cell line HeLa and in preliminary testing of U251 cell lysate (calculated MW of 42.8kDa according to NP_009213.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC