Goat Anti-Robo3.2 (mouse aa1303-1316) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

roundabout homolog 3 (Drosophila)|roundabout homolog 3|Robo3b|Robo3a|Robo3|Rig-1|Rig1|retinoblastoma-inhibiting gene 1 protein|retinoblastoma inhibiting gene 1|Rbig1

AAD11628.1

EBP11776

Peptide with sequence KESQGRGRGLEACR, from the C-terminus of the protein sequence according to AAD11628.1.

KESQGRGRGLEACR

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human

19649

Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.

Antibody detection limit dilution 1:128000.

Preliminary experiments gave bands at approx 85kDa in Mouse fetal Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa according to Mouse AAD11628.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Pep-ELISA, IHC