Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names OTTHUMP00000028981|SH3 domain binding protein 1|SH3-domain binding protein 1|SH3BP1 | |
Usage Summary Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press). | |
Accession ID NP_061830.3 | |
Blocking Peptide EBP06271 | |
Immunogen Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3. | |
Peptide Sequence C-PQPRPRSLASETN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 23616 | |
Product Grade  | |
IHC Results In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 70kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7kDa according to NP_061830.3). Recommended for use at 1-3µg/ml. | |
Application Type Pep-ELISA, WB, IHC, ICC, IF |
Goat Anti-SH3BP1 Antibody
$464.00
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Selected References [{"pmid": 21658605, "intro": "This antibody has been successfully used in Time-lapse microscopy on Rat:", "title": "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility.", "author": "Parrini MC, Sadou-Dubourgnoux A, Aoki K, Kunida K, Biondini M, Hatzoglou A, Poullet P, Formstecher E, Yeaman C, Matsuda M, Rossé C, Camonis J.", "journal": "Mol Cell. 2011 Jun 10;42(5):650-61."}] |