Goat Anti-SMC5 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09424
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
SMC5 structural maintenance of chromosomes 5-like 1|SMC5 protein|OTTHUMP00000063367|SMC5L1|KIAA0594|structural maintenance of chromosomes 5|SMC5
Usage Summary
Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_055925.2
Blocking Peptide
EBP09424
Immunogen
Peptide with sequence C-TSKHLEHKDNELRQ, from the Internal region of the protein sequence according to NP_055925.2.
Peptide Sequence
C-TSKHLEHKDNELRQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow, Dog
Reactive Species
Human
Human Gene ID
23137
Mouse Gene ID
226026
Rat Gene ID
293967
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary testing showed bands at approx 100+150kDa in HEK293 nuclear lysates after 1µg/ml antibody staining (calculated MW of 129kDa according to NP_055925.2). These bands were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC

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