Goat Anti-SORBS1/ponsin (internal region) Antibody

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SKU: EB07687
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ponsin|c-Cbl associated protein|SH3-domain protein 5 (ponsin)|SH3-domain protein 5|Fas-ligand associated factor 2|SORB1|SH3P12|SH3D5|R85FL|KIAA1296|FLJ12406|FLAF2|DKFZp586P1422|DKFZp451C066|CAP|sorbin and SH3 domain containing 1|SORBS1
Accession ID
NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1; NP_001277225.2; NP_001364131.1; NP_001371378.1
Blocking Peptide
EBP07687
Immunogen
Peptide with sequence C-SERNDWEPPDKKVD, from the internal region of the protein sequence according to NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1; NP_001277225.2; NP_001364131.1; NP_001371378.1.
Product Comments
This antibody is expected to recognise multiple isoforms.
Peptide Sequence
C-SERNDWEPPDKKVD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
10580
Mouse Gene ID
20411
Product Grade
IHC Results
Paraffin embedded Human Pancreas and Liver. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 65kDa band in Human Adipose lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6kDa according to NP_006425.2 and higher sizes for other isoforms. The 65kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC