Goat Anti-SOX2 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07378
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
transcription factor SOX2|sex-determining region Y-box 2|SRY-related HMG-box gene 2|MGC2413|ANOP3|SRY (sex determining region Y)-box 2|SOX2
Usage Summary
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_003097.1
Blocking Peptide
EBP07378
Immunogen
Peptide with sequence YNMMETELKPPGPQQ-C, from the N Terminus of the protein sequence according to NP_003097.1.
Peptide Sequence
YNMMETELKPPGPQQ-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
6657
Product Grade
IHC Results
Paraffin embedded Human Tonsil. Recommended concentration: 6-8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 40kDa band observed in HEK293 and MCF7 cell lysates and in nuclear lysates of cell line MCF7 (calculated MW of 34.3kDa according to )NP_003097.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, FC, IHC

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