Goat Anti-SPAG16 (aa139-152) Antibody

$464.00

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SKU: EB09826
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
SPAG16|WDR29|WD repeat domain 29|sperm-associated WD repeat protein|sperm associated antigen 16|PF20|MGC87036|FLJ37717|FLJ22724|DKFZp666P1710
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_078808.3; NP_001020607.1
Blocking Peptide
EBP09826
Immunogen
Peptide with sequence C-KGVTELRTVGNVPD, from the internal region of the protein sequence according to NP_078808.3; NP_001020607.1.
Product Comments
This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).
Peptide Sequence
C-KGVTELRTVGNVPD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Cow
Reactive Species
Human, Mouse
Human Gene ID
79582
Product Grade
IHC Results
This antibody was successfully used in Human and Murine frozen Brain sections (PMID: 25086173).
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Application Type
Pep-ELISA, FC, IF, IHC
Selected References
[{"pmid": 25086173, "intro": "", "title": "Sperm-associated antigen 16 is a novel target of the humoral autoimmune response in multiple sclerosis.", "author": "de Bock L, Somers K, Fraussen J, Hendriks JJ, van Horssen J, Rouwette M, Hellings N, Villar LM, Alvarez-Cermeño JC, Espiño M, Hupperts R, Jongen P, Damoiseaux J, Verbeek MM, De Deyn PP, D'hooghe M, Van Wijmeersch B, Stinissen P, Somers V.", "journal": "J Immunol. 2014 Sep 1;193(5):2147-56."}]