Goat Anti-Sprouty Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)|sprouty (Drosophila) homolog 1 (antagonist of FGF signaling)|sprouty homolog 1, antagonist of FGF signaling (Drosophila)|hSPRY1|SPRY1

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

NP_005832.1; NP_955359.1

EBP06432

Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1; NP_955359.1.

NP_005832.1 and NP_955359.1 represent varients of the same protein.

CPSRGQGKPS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human, Mouse

10252

This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3µg/ml.

Antibody detection limit dilution 1:16000.

Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162). Recommended concentration: 1-3µg/ml.

Pep-ELISA, WB, IF, FC, IHC