Goat Anti-STAT3 Antibody

$464.00

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SKU: EB07379
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
signal transducer and activator of transcription 3|acute-phase response factor|DNA-binding protein APRF|MGC16063|FLJ20882|APRF|signal transducer and activator of transcription 3 (acute-phase response factor)|STAT3
Usage Summary
Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml
Accession ID
NP_644805.1; NP_003141.2; NP_998827.1
Blocking Peptide
EBP07379
Immunogen
Peptide with sequence CRPESQEHPEADP, from the internal region of the protein sequence according to NP_644805.1; NP_003141.2; NP_998827.1.
Product Comments
This antibody is expected to recognise all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).
Peptide Sequence
CRPESQEHPEADP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Mouse
Human Gene ID
6774
Mouse Gene ID
20848
Rat Gene ID
25125
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 85kDa band observed in lysates of cell line LNCaP, and approx. 90kDa in Mouse Heart lysates (calculated MW of 83.1kDa according to Human NP_998827.1 and 88.1kDa according to Mouse : NP_998824.1). Recommended concentration: 0.5-3ug/ml. Primary incubation 1 hour. at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC