Goat Anti-Sterol carrier protein 2 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

SCP2|sterol carrier protein 2|DKFZp686C12188|DKFZp686D11188|NLTP|NSL-TP|SCPX|nonspecific lipid-transfer protein|sterol carrier protein X

NP_001007099.1; NP_002970.2; NP_001180528.1; NP_001180529.1; NP_001180546.1

EBP08546

Peptide with sequence C-KNHKHSVNNPYSQ, from the internal region of the protein sequence according to NP_001007099.1; NP_002970.2; NP_001180528.1; NP_001180529.1; NP_001180546.1.

This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 (NP_002970.2; NP_001007099.1; NP_001180529.1; NP_001180528.1; NP_001180546.1 respectively),

C-KNHKHSVNNPYSQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human

6342

20280

25541

In paraffin embedded Human Liver shows speckled cytoplasm staining in hepatocytes. Recommended concentration, 3-6µg/ml.

Antibody detection limit dilution 1:32000.

Preliminary experiments gave an approx 50kDa band in Human Liver lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.0kDa according to NP_001007099.1 and of 6.7kDa according to NP_002970.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC