Goat Anti-TNNT3 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB12033
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
troponin-T3, skeletal, fast|troponin T, fast skeletal muscle|fast skeletal muscle troponin T|fTnT|beta-TnTF|beta TnTF|TNTF|troponin T type 3 (skeletal, fast)|TNNT3
Accession ID
NP_006748.1; NP_001036246.1; NP_001036247.1
Blocking Peptide
EBP12033
Immunogen
Peptide with sequence C-TTLRSRIDQAQKHSK, from the C Terminus of the protein sequence according to NP_006748.1; NP_001036246.1; NP_001036247.1.
Product Comments
This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1). Reported variants represent identical protein: NP_001284575.1, NP_001036247.1
Peptide Sequence
C-TTLRSRIDQAQKHSK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human, Mouse, Rat
Human Gene ID
7140
Mouse Gene ID
21957
Rat Gene ID
24838
Product Grade
IHC Results
In paraffin embedded Human Skeletal Muscle shows staining of select fibrils. Recommended concentration, 4-8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 35kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et al, Circ Res. 1997 Jul;81(1):76-85. PMID: 9201030.).Recommended concentration: 0.01-0.03µg/ml.
Application Type
Pep-ELISA, WB, IHC

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