Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names target of myb1-like 1|target of myb 1-like 1|Src activating and signaling molecule|target of myb1 (chicken)-like 1|SRCASM|TOM1L1 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_005477.2; NP_001308102.1; NP_001308103.1 | |
Blocking Peptide EBP08388 | |
Immunogen Peptide with sequence C-EQNKNQKEATNTTSE, from the internal region of the protein sequence according to NP_005477.2; NP_001308102.1; NP_001308103.1. | |
Peptide Sequence C-EQNKNQKEATNTTSE | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 10040 | |
Product Grade ![]() | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot This antibody was observed to recognize variations of bands/isoforms at approx. 37kDa, 45kDa, 50kDa, 55kDa and 60kDa in lysates of cell lines HEK293, A431 and HeLa, and in Human Cerebellum lysates (calculated MW of 38.7kDa according to NP_001308102.1, 44.3kDa according to NP_001308103.1 and 53.0kDa according to NP_005477.2). All bands were blocked by incubation with the immunizing peptide; please refer to WB images for details. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.
Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-TOM1L1 / SRCASM Antibody
$464.00
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