Goat Anti-TOM1L1 / SRCASM Antibody

$464.00

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SKU: EB08388
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
target of myb1-like 1|target of myb 1-like 1|Src activating and signaling molecule|target of myb1 (chicken)-like 1|SRCASM|TOM1L1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_005477.2; NP_001308102.1; NP_001308103.1
Blocking Peptide
EBP08388
Immunogen
Peptide with sequence C-EQNKNQKEATNTTSE, from the internal region of the protein sequence according to NP_005477.2; NP_001308102.1; NP_001308103.1.
Peptide Sequence
C-EQNKNQKEATNTTSE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
10040
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
This antibody was observed to recognize variations of bands/isoforms at approx. 37kDa, 45kDa, 50kDa, 55kDa and 60kDa in lysates of cell lines HEK293, A431 and HeLa, and in Human Cerebellum lysates (calculated MW of 38.7kDa according to NP_001308102.1, 44.3kDa according to NP_001308103.1 and 53.0kDa according to NP_005477.2). All bands were blocked by incubation with the immunizing peptide; please refer to WB images for details. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.

Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.

Application Type
Pep-ELISA, WB, IF, FC