Goat Anti-TRIM2 Antibody

$464.00

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SKU: EB05914
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
tripartite motif protein TRIM2|RNF86|tripartite motif protein TRIM2|tripartite motif protein 2|KIAA0517|tripartite motif-containing 2|TRIM2
Usage Summary
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Accession ID
NP_056086.2; NP_001123539.1; NP_001337983.1; NP_001337984.1; NP_001337985.1l; NP_001337986.1
Blocking Peptide
EBP05914
Immunogen
Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2; NP_001123539.1; NP_001337983.1; NP_001337984.1; NP_001337985.1l; NP_001337986.1.
Product Comments
Cross-reactivity with TRIM3 has been demonstrated in Mouse.
Peptide Sequence
DSGNHCFKVYRYLQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Rat
Human Gene ID
23321
Mouse Gene ID
80890
Product Grade
IHC Results
In paraffin embedded Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies. Recommended concentration: 6-12µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 75-85kDa band observed in Rat Brain lysates (calculated MW of 83.3 according to Rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC