Goat Anti-TRIM29 / ATDC Antibody

$464.00

Category: Primary Antibodies
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SKU: EB09814
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
TRIM29|tripartite motif-containing 29|tripartite motif protein TRIM29|FLJ36085|ATDC|ataxia-telangiectasia group D-associated protein
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_036233.2; NP_001317311.1
Blocking Peptide
EBP09814
Immunogen
Peptide with sequence C-EKDRIKSFTTNE, from the internal region of the protein sequence according to NP_036233.2; NP_001317311.1.
Peptide Sequence
C-EKDRIKSFTTNE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human
Human Gene ID
23650
Mouse Gene ID
72169
Rat Gene ID
300656
Product Grade
IHC Results
In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 37.3kDa according to NP_001317311.1 (isoform 2) and 65.8kDa according to NP_036233.2 (isoform 1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room tempetature.
Application Type
Pep-ELISA, WB, IF, FC, IHC

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