Goat Anti-VPS29 (Internal) Antibody

$423.00

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SKU: EB07369
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
x 007 protein|vacuolar sorting protein VPS29/PEP11|vacuolar protein sorting 29 (yeast homolog)|vacuolar protein sorting 29|retromer protein|PEP11|FLJ20492|DKFZp564F0223|DC7|DC15|vacuolar protein sorting 29 (yeast)|VPS29
Accession ID
NP_057310.1; NP_476528.1
Blocking Peptide
EBP07369
Immunogen
Peptide with sequence CTGNLCTKESYDYLK, from the internal region of the protein sequence according to NP_057310.1; NP_476528.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_057310.1 and NP_476528.1).
Peptide Sequence
CTGNLCTKESYDYLK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
51699
Mouse Gene ID
56433
Rat Gene ID
288666
Product Grade
IHC Results
Paraffin embedded Human Uterus and Placenta. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC