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Do you wish immunofluorescence of suspension cells was as easy as with adherent cells? Now it is! Shi-fix™ (manufactured by Shikhar Biotech) cover-slips allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining (or continue the culture to obtain desired cell densities).
These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. You can work on non-adherent cells as if they were adherent!
For the differences between Shi-fix and Shi-fix Plus please see our technical support page here.
Please find the datasheet for all Shi-fix products here.
Suspension cell immunofluorescence with Shi-fix™ coverslips
Shi-fix coverslips have been successfully used in the following papers:
Hiroya Shiba et al. (2022) Carboxy-terminal dendrimers with phenylalanine for a pH-sensitive delivery system into immune cells including T cells. J Mater Chem B. 2022 Apr 6;10(14):2463-2470. PMID: 34935852.
Jeffrey R Whiteaker et al. (2021) Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Cancers (Basel). 2021 Jul 30;13(15):3843. PMID: 34359745.
Rima Kandil et al. (2019) Coming in and Finding Out: Blending Receptor-Targeted Delivery and Efficient Endosomal Escape in a Novel Bio-Responsive siRNA Delivery System for Gene Knockdown in Pulmonary T Cells. Adv Ther (Weinh). 2019 Jul;2(7):1900047. PMID: 31372493.
Servando Hernandez Vargas et al. (2019) Specific Targeting of Somatostatin Receptor Subtype-2 for Fluorescence-Guided Surgery. Clin Cancer Res. 2019 Jul 15;25(14):4332-4342. PMID: 31015345.