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Goat Anti-BPOZ / ABTB1 Antibody (EB05200)

Related Products: EBP05200 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05200 Goat Anti-BPOZ / ABTB1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05200 1
EB05200 staining (1µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05200 2
EB05200 (3.75µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05200 3
EB05200 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABTB1, BPOZ, EF1ABP, MGC20585, ankyrin repeat and BTB (POZ) domain containing 1, elongation factor 1A binding protein, Btb3, PP2259
Official Symbol: ABTB1
Accession Number(s): NP_115937.1; NP_742024.1
Human GeneID(s): 80325
Non-Human GeneID(s): 80283 (mouse)
Important Comments: This antibody is expected to recognise all reported isoforms (NP_115937.1; NP_742024.1; NP_742025.1).

Immunogen

Peptide with sequence RALEDLLVSIGLDC, from the C Terminus of the protein sequence according to NP_115937.1; NP_742024.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38kDa band observed in Human Heart lysates, and approx. 50kDa in preliminary testing of MCF7 cell lysate (calculated MW of 37.6kDa according to NP_115937.1 and 54kDa according to ; NP_742024.1). An additional band was also consistently observed at 65-70xkDa. and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Heart and Brain (Cerebellum). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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