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Goat Anti-ATF4 Antibody (EB05247)

Related Products: EBP05247 - Immunizing Peptide, Goat Anti-ATF4 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05247 Goat Anti-ATF4 Antibody Pep-ELISA, WB Allow up to 10 days for delivery 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05247 1
EB05247 (1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATF4, activating transcription factor 4 (tax-responsive enhancer element B67), CREB2, TXREB, CREB-2, TAXREB67, OTTHUMP00000199130, activating transcription factor 4, cAMP response element-binding protein 2
Official Symbol: ATF4
Accession Number(s): NP_001666.2; NP_877962.1
Human GeneID(s): 468
Non-Human GeneID(s): 11911 (mouse), 79255 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).

Immunogen

Peptide with sequence C-EEVRKARGKKRVP, from the C Terminus of the protein sequence according to NP_001666.2; NP_877962.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 35-37kDa band observed in Mouse Heart lysates (calculated MW of 38.3kDa according to Mouse NP_001274109.1; NP_033846.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Pig

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