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Code Name Applications Availability Size Tested species Price Grade  
EB05267 Goat Anti-CDCP1 (isoform 1: C term) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05267 1
EB05267 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05267 2
EB05267 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDCP1, CUB domain containing protein 1, HGNC:24357, CD318, SIMA135, TRASK, CUB domain-containing protein 1, transmembrane and associated with src kinases
Official Symbol: CDCP1
Accession Number(s): NP_073753.3
Human GeneID(s): 64866
Important Comments: This antibody is expected to recognize isoform 1 (NP_073753.3) only.

Immunogen

Peptide with sequence C-PLLNTQEPMEPAE, from the C Terminus of the protein sequence according to NP_073753.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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