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Code Name Applications Availability Size Tested species Price Grade  
EB05307 Goat Anti-Annexin A11 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05307 1
EB05307 (0.01µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05307 2
EB05307 (3.8µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ANXA11, annexin A11, ANX11, CAP50, annexin XI, autoantigen, 56-kD, calcyclin-associated annexin 50, 56 kDa autoantigen, CAP-50, annexin-11
Official Symbol: ANXA11
Accession Number(s): NP_001148.1
Human GeneID(s): 311
Important Comments: Reported variants represent identical protein: NP_001148.1; NP_665875.1 and NP_665876.1.

Immunogen

Peptide with sequence YPGYPPPPGGYPP-C, from the N Terminus of the protein sequence according to NP_001148.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in lysates of cell lines HeLa and Jurkat (calculated MW of 54.4kDa according to NP_001148.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: In paraffin embedded Human Placenta shows strong staining of Basement Membrane in trophoblasts. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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