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Goat Anti-Annexin A2 Antibody (EB05310)

Related Products: EBP05310 - Immunizing Peptide, Goat Anti-Annexin A2 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05310 Goat Anti-Annexin A2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05310 1
EB05310 staining (0.5µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ANXA2, annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, annexin II, lipocortin II, Annexin II (lipocortin I), annexin II (lipocortin II), calpactin I, heavy polypeptide (p36), annexin II (lipocortin II calpactin I, heavy polypeptide), calpactin I heavy polypeptide, chromobindin 8, lipocortin II, placental anticoagulant protein IV, HEL-S-270
Official Symbol: ANXA2
Accession Number(s): NP_001002858.1; NP_004030.1
Human GeneID(s): 302
Non-Human GeneID(s): 12306 (mouse), 56611 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001002858.1; NP_004030.1). Variants NP_004030.1, NP_001002857.1 and NP_001129487.1 represent identical protein.

Immunogen

Peptide with sequence STVHEILCKLSLEGD, from the N Terminus of the protein sequence according to NP_001002858.1; NP_004030.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 35kDa band observed in lysates of cell lines A549, HeLa and NIH3T3 (calculated MW of 38.6kDa according to NP_004030.1 and NP_001002858.1). Recommended concentration: 0.5-3µg/ml. Primary incubation was for 1 hour.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig

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