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Goat Anti-RAC2 Antibody (EB05314)

Related Products: EBP05314 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05314 Goat Anti-RAC2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05314 1
EB05314 (0.1µg/ml) staining of Molt4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05314 2
EB05314 (2µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RAC2, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2), EN-7, Ras-related C3 botulinum toxin substrate 2, rho family, small GTP binding protein Rac2, Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2), Gx, HSPC022, small G protein
Official Symbol: RAC2
Accession Number(s): NP_002863.1
Human GeneID(s): 5880

Immunogen

Peptide with sequence PQPTRQQKRACSLL, from the C Terminus of the protein sequence according to NP_002863.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 22kDa band observed in lysates of cell line Molt4 (calculated MW of 21.4kDa according to NP_002863.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Tonsil shows staining in mantle zone and some in the interfolicular area. Unserstained in germinal centre. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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