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Code Name Applications Availability Size Tested species Price Grade  
EB05315-B Goat Anti-RACGAP1 / MgcRacGAP, Biotinylated Antibody Pep-ELISA, WB, IHC Contact sales for delivery estimate 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05315-B 1
Biotinylated EB05315 (1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: RACGAP1, Rac GTPase activating protein 1, CYK4, HsCYK-4, ID-GAP, MgcRacGAP, male germ cell RacGap, protein CYK4 homolog
Official Symbol: RACGAP1
Accession Number(s): NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1
Human GeneID(s): 29127
Important Comments: This antibody is expected to recognize all reported isoforms (NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_001119575.1, NP_001306932.1, NP_001306930.1, NP_001306933.1

Immunogen

Peptide with sequence CGRQGNFFASPMLK., from the C Terminus of the protein sequence according to NP_037409.2; NP_001306934.1; NP_001306935.1; NP_001306936.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 75kDa band observed in lysates of cell line Jurkat (calculated MW of 71.0kDa according to NP_037409.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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