Change country
Home

Goat Anti-VAV2 Antibody (EB05337)

Related Products: EBP05337 - Immunizing Peptide, Goat Anti-VAV2 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05337 Goat Anti-VAV2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05337 1
EB05337 staining (1.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05337 2
EB05337 (2.5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: VAV2, vav 2 oncogene, Oncogene VAV2, vav 2 guanine nucleotide exchange factor, Protein vav-2, oncogene VAV2
Official Symbol: VAV2
Accession Number(s): NP_001127870.1; NP_003362.2
Human GeneID(s): 7410
Non-Human GeneID(s): 22325 (mouse)
Important Comments: This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2).

Immunogen

Peptide with sequence ETEAKYYRTLEDIEC, from the internal region of the protein sequence according to NP_001127870.1; NP_003362.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100kDa band observed in 293 lysate (predicted MW of 100kDa according to NP_003362). Recommended for use at 1-1.5µg/ml.
IHC: Paraffin embedded Human Colon. Recommended concentration: 2.5µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

Product Reviews

Please login to review this product.