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Goat Anti-Septin 3 Antibody (EB05413)

Related Products: EBP05413 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05413 Goat Anti-Septin 3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05413 1
EB05413 (0.5µg/ml) staining of Human Cerebellum (A), (0.1ug/ml) Mouse (B) and (0.03ug/ml) Rat (C) Brain lysate (35µg protein in RIPA buffer).. Detected by chemiluminescence.
EB05413 2
EB05413 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05413 3
EB05413 (3.75µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05413 4
EB05413 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SEPT3, septin 3, SEP3, CTA-250D10.3, MGC133218, bK250D10.3, OTTHUMP00000028742, neuronal-specific septin 3, G-Septin
Official Symbol: SEPT3
Accession Number(s): NP_663786.2; NP_061979.3
Human GeneID(s): 55964
Non-Human GeneID(s): 24050 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms according to NP_663786.2 and NP_061979.3.

Immunogen

Peptide with sequence SELVPEPRPKPA-C, from the N Terminus of the protein sequence according to NP_663786.2; NP_061979.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in Human Cerebellum and in Mouse and Rat Brain lysates (calculated MW of 40.7kDa according to Human NP_663786.2 and Rat NP_062248.2, and 40.6kDa according to Mouse NP_001345765.1). Recommended concentration: 0.03-0.5ug/ml. Primary incubation 1 hour at room temperature
IHC: Paraffin embedded Human Brain (Cortex) and (Cerebellum) and Testis. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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