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Goat Anti-RGS1 / 1R20 Antibody (EB05421)

Related Products: EBP05421 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05421 Goat Anti-RGS1 / 1R20 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05421 1
EB05421 staining (1µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05421 2
EB05421 (2.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05421 3
EB05421 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RGS1, 1R20, regulator of G-protein signalling 1, BL34, IER1, IR20, 1R20, Regulator of G-protein signaling 1, B-cell activation protein BL34, early response protein 1R20, immediate-early response 1, B-cell specific
Official Symbol: RGS1
Accession Number(s): NP_002913.3
Human GeneID(s): 5996
Non-Human GeneID(s): 50778 (mouse)

Immunogen

Peptide with sequence C-NLLNDLQANSLK, from the C Terminus of the protein sequence according to NP_002913.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 25kDa band seen in HepG2 lysate. Recommended for use at 0.5-2µg/ml.
IHC: Paraffin embedded Human Thymus and Small Intestine. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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