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Goat Anti-KIF4A Antibody (EB05480)

Related Products: EBP05480 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05480 Goat Anti-KIF4A Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05480 1
EB05480 (2µg/ml) staining of nuclear NIH3T3 (A) and (2ug/ml) negative control Human Ovary (B) lysate. (35µg protein in RIPA buffer) Detected by chemiluminescence.
EB05480 2
EB05480 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB05480 3
EB05480 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: KIF4A, kinesin family member 4A, FLJ12530, FLJ12655, FLJ14204, FLJ20631, HSA271784, KIF4, KIF4-G1, chromokinesin, kinesin family member 4
Official Symbol: KIF4A
Accession Number(s): NP_036442.3
Human GeneID(s): 24137

Immunogen

Peptide with sequence SFFSGCSPIEEEAH, from the C Terminus of the protein sequence according to NP_036442.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 150kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5kDa according to Mouse NP_032472.2). Recommended concentration:1-2µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Ovary lysate.

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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