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Goat Anti-PAX8 (N Terminus) Antibody (EB05513)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05513 | Goat Anti-PAX8 (N Terminus) Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human | $411.00 |  |
|
Ordering Information forOrder direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. Order offline99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:
In stock orders received by 11 am PST on Monday through Friday are shipped the same day. Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. |
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Target Protein
Principal Names: PAX8, paired box gene 8, paired domain gene 8, paired box 8Official Symbol: PAX8
Accession Number(s): NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1
Human GeneID(s): 7849
Non-Human GeneID(s): 18510 (mouse), 81819 (rat)
Important Comments: This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Immunogen
Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 35kDa band observed in Human Kidney lysates. An additional 48kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9kDa according to Human NP_039247.1 PAX8D and 48.2kDa according to Human NP_003457.1 PAXA). Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Liver, and NIH3T3 for this particular batch.
IHC: Paraffin embedded Human Kidney Cortex. Recommended concentration: 5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat
Product Reviews
Please login to review this product.- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol
FAQs
Message from a distributor:
Please could you assist with this technical issue. The customer saw positive staining in the membrane of catalog# EB05513 . Please could you suggest a cause of this. Can you recommend a dilution and enzyme digestion?
Our EB05513 reacts with transcription factor PAX8 and it should be staining the nuclei. This is exactly what we have found in Human Thyroid Gland. It is possible however that this product does not work properly after different epitope retrieval methods. We obtained our IHC data after microwaved antigen retrieval with Tris/EDTA at pH9. A different pH value or a different heating type may result in different results.
I hope this will help your customer further. Please let me know when problems persist after adjustment of the epitope retrieval method.