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Goat Anti-Aldehyde Reductase Antibody (EB05538)

Related Products: EBP05538 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05538 Goat Anti-Aldehyde Reductase Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05538 1
EB05538 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05538 2
EB05538 (5µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05538 3
EB05538 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: aldehyde reductase, AKR1A1, aldo-keto reductase family 1, member A1 (aldehyde reductase), ALDR1, ALR, MGC12529, MGC1380, alcohol dehydrogenase, aldo-keto reductase family 1, member A1, DD3, OTTHUMP00000009240, dihydrodiol dehydrogenase 3
Official Symbol: AKR1A1
Accession Number(s): NP_006057.1; NP_697021.1
Human GeneID(s): 10327
Non-Human GeneID(s): 58810 (mouse), 78959 (rat)
Important Comments: Both reported variants (NP_006057.1and NP_697021.1) represent identical protein

Immunogen

Peptide with sequence C-DAGHPLYPFNDPY, from the C Terminus of the protein sequence according to NP_006057.1; NP_697021.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 37kDa band observed in Human Liver lysates (calculated MW of 36.6kDa according to NP_006057.1 and NP_697021.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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