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Code Name Applications Availability Size Tested species Price Grade  
EB05676 Goat Anti-BOB1 / OCAB / OBF1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05676 1
EB05676 (0.3µg/ml) staining of Human (A), Mouse (B) and Rat (C) Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05676 2
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human BOB1 with DYKDDDDK tag probed with EB05676(1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB05676(1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BOB1, OBF1, POU2AF1, OBF-1, POU domain, class 2, associating factor 1, POU class 2 associating factor 1, OCAB
Official Symbol: POU2AF1
Accession Number(s): NP_006226.2
Human GeneID(s): 5450
Non-Human GeneID(s): 18985 (mouse)

Immunogen

Peptide with sequence C-DAYALNHTLSVEGF, from the C Terminus of the protein sequence according to NP_006226.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 30kDa band seen in mouse spleen lysate (Predicted MW of 28kDa according to NP_006226). Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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