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Goat Anti-HIP2 Antibody (EB05750)

Related Products: EBP05750 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05750 Goat Anti-HIP2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05750 1
EB05750 (3.8µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05750 2
EB05750 staining (1.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBE2K, HIP2, huntingtin interacting protein 2, LIG, HYPG, ubiquitin-protein ligase, ubiquitin carrier protein, ubiquitin-conjugating enzyme E2-25 KDA, ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast), DKFZp564C1216, DKFZp686J24237, E2-25K
Official Symbol: UBE2K
Accession Number(s): NP_005330.1; NP_001104582.1; NP_001104583.1
Human GeneID(s): 3093
Important Comments: This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).

Immunogen

Peptide with sequence C-SWDVETATELLLSN, from the C Terminus of the protein sequence according to NP_005330.1; NP_001104582.1; NP_001104583.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 24kDa band observed in Jurkat cell lysates (calculated MW of 22.4 kDa according to NP_005330). Recommended for use at 1-3 µg/ml.
IHC: In paraffin embedded Human Brain shows pixulate cytoplasm stainng in neurons of the Cortex. Recommended concentration, 1-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow

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