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Goat Anti-ARIH2 / TRIAD1 Antibody (EB05813)

Related Products: EBP05813 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05813 Goat Anti-ARIH2 / TRIAD1 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05813 1
EB05813 (2µg/ml) staining of Hela (A) and K562 (B) nuclear lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2
Official Symbol: ARIH2
Accession Number(s): NP_006312.1
Human GeneID(s): 10425
Non-Human GeneID(s): 23807 (mouse)

Immunogen

Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 60kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8kDa according to NP_006312.1) Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8kDa according to NP_006312.1). Recommended concentration: 1-3µg/ml. This antibody has also been successfully used in WB in PMID: 24486325.

Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in WB and IF on Human:
Raz V, Buijze H, Raz Y, Verwey N, Anvar SY, Aartsma-Rus A, van der Maarel SM.
A novel feed-forward loop between ARIH2 E3 ligase and PABPN1 regulates aging-associated muscle degeneration.
Am J Pathol. 2014 Apr;184(4):1119-31.
PMID: 24486325

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