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Goat Anti-RNF3 (247 aa isoform) Antibody (EB05837)

Related Products: EBP05837 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05837 Goat Anti-RNF3 (247 aa isoform) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05837 1
EB05837 staining (1µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: RNF3, DONG1, RNF3A, MGC40413, ring finger protein 3, PCGF3, polycomb group ring finger 3, DKFZp686D20235, FLJ36550, FLJ43813, MGC129615
Official Symbol: PCGF3
Accession Number(s): NP_006306.2
Human GeneID(s): 10336
Non-Human GeneID(s): 69587 (mouse)
Important Comments: This antibody is expected to recognise the 247 aa isoform only (NP_006306), not the 132 aa isoform (NP_694982).

Immunogen

Peptide with sequence C-PLLLHYRPKMDLL, from the C Terminus of the protein sequence according to NP_006306.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 35kDa band seen in Human Heart lysate [Predicted MW of approx. 27kDa according to NP_006306]. Recommended for use at 0.5-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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