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Goat Anti-RNF8 Antibody (EB05843)

Related Products: EBP05843 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05843 Goat Anti-RNF8 Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05843 1
EB05843 (1µg/ml) staining of HepG2 (A) and Jurkat (B) nuclear cell lysate. (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05843 2
EB05843 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: RNF8, ring finger protein 8, ring finger protein (C3HC4 type) 8, KIAA0646, FLJ12013, C3HC4-type zinc finger protein, OTTHUMP00000039684, UBC13/UEV-interacting ring finger protein
Official Symbol: RNF8
Accession Number(s): NP_003949.1; NP_898901.1
Human GeneID(s): 9025
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).

Immunogen

Peptide with sequence GEPGFFVTGDRAG-C, from the N Terminus of the protein sequence according to NP_003949.1; NP_898901.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in Human Testis and Lung lysates and approx. 50kDa in nuclear lysates of cell lines HepG2 and Jurkat (predicted MW of 56kDa according to NP_003949.1 and 50.8kDa according to NP_898901.1 ). Recommended concentration 0.5-2µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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