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Goat Anti-RNF36 / TRIM69 Antibody (EB05894)

Related Products: EBP05894 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05894 Goat Anti-RNF36 / TRIM69 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05894 1
EB05894 (0.5µg/ml) staining of Mouse Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05894 2
EB05894 (2.5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05894 3
EB05894 (2.5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM69, tripartite motif-containing 69, HSD34, RNF36, Trif, ring finger protein 36, trimless, tripartite motif protein 69, hCG_39321, RNF36
Official Symbol: TRIM69
Accession Number(s): NP_892030.3; NP_542783.2
Human GeneID(s): 140691
Important Comments: This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2).

Immunogen

Peptide with sequence C-GENKEPLHILHPQ, from the C Terminus of the protein sequence according to NP_892030.3; NP_542783.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in Mouse Testis lysates (calculated MW of 57.3kDa according to Mouse NP_536771.2). Recommended concentration: 0.5-2µg/ml.
IHC: Paraffin embedded Human Testis and Brain (Cerebellum). Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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