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Goat Anti-TRIM4 Antibody (EB05931)

Related Products: EBP05931 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05931 Goat Anti-TRIM4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05931 1
EB05931 staining (1µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM4, RNF87, tripartite motif-containing 4, tripartite motif protein 4, tripartite motif protein TRIM4, tripartite motif protein TRIM4 isoform alpha, tripartite motif protein 4
Official Symbol: TRIM4
Accession Number(s): NP_148977.1; NP_149082.1
Human GeneID(s): 89122
Important Comments: This antibody is expected to recognise both reported human isoforms (as represented by NP_148977 and NP_149082).

Immunogen

Peptide with sequence C-ASLVIPPVTDRK, from the C Terminus of the protein sequence according to NP_148977.1; NP_149082.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55-60kDa band observed in HepG2 whole cell lysate (predicted MW of 58kDa and 55kDa according to NP_148977 and NP_149082). Recommended for use at 1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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