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Goat Anti-Pallidin Antibody (EB05946)

Related Products: EBP05946 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05946 Goat Anti-Pallidin Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05946 1
EB05946 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05946 2
HEK293 overexpressing PLDN (RC201514) and probed with EB05946 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: pallidin homolog (mouse), pallidin, PLDN, PA, PALLID, pallid (mouse) homolog, pallidin, syntaxin 13 binding protein 1, syntaxin 13-interacting protein pallid
Official Symbol: BLOC1S6
Accession Number(s): NP_036520.1
Human GeneID(s): 26258

Immunogen

Peptide with sequence C-EREKQLTARPAKRM, from the C Terminus of the protein sequence according to NP_036520.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 23-24kDa band observed in lysates of cell line A431 (calculated MW of 19.7kDa according to NP_036520.1). In transfected HEK293 transiently expressing PLDN a band of approx. 28kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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