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Goat Anti-Coronin 1 / TACO Antibody (EB06057)

Related Products: EBP06057 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06057 Goat Anti-Coronin 1 / TACO Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06057 1
EB06057 (0.5µg/ml) staining of Daudi (A), MOLT4 (B) and (1ug/ml) of Jurkat (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06057 2
EB06057 (0.5µg/ml) staining of Human Lymph node (A) and Spleen (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06057 3
EB06057 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06057 4
EB06057 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06057 5
EB06057 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CORO1A, Coronin 1, TACO, p57, CLABP, HCORO1, CLIPINA, Clabp TACO, coronin, actin binding protein, 1A, coronin-1, coronin, actin-binding, 1A, coronin, actin-binding protein, 1A, FLJ41407, MGC117380
Official Symbol: CORO1A
Accession Number(s): NP_009005.1
Human GeneID(s): 11151
Non-Human GeneID(s): 12721 (mouse), 155151 (rat)

Immunogen

Peptide with sequence C-KRLDRLEETVQAK, from the C Terminus of the protein sequence according to NP_009005.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 60kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 and approx. 55kDa in Human Lynph node and Spleen lysates (calculated MW of 51.0kDa according to Human NP_009005.1). These molecular weights are routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Spleen and Small Intestine. Recommended concentration: 3.75µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody (previous batch) has been successfully used in WB on Mouse:
Honoré B, Buus S, Claësson MH.
Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53.
Proteome Sci. 2008 Jun 10;6:18.
PMID: 18544163

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