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Goat Anti-EPS8 Antibody (EB06081)

Related Products: EBP06081 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06081 Goat Anti-EPS8 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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  • Purchase order number
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06081 1
EB06081 (1µg/ml) staining of lysates of cell line NIH3T3 (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06081 2
EB06081 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06081 3
EB06081 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
EB06081 4
EB06081 (8µg/ml) staining of paraffin embedded Human Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06081 5
EB06081 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: EPS8, epidermal growth factor receptor pathway substrate 8
Official Symbol: EPS8
Accession Number(s): NP_004438.3
Human GeneID(s): 2059
Non-Human GeneID(s): 13860 (mouse)

Immunogen

Peptide with sequence C-SGVESFDEGSSH, from the C Terminus of the protein sequence according to NP_004438.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 91.9kDa according to NP_004438.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney and Pancreas. Recommended concentration: 6-8µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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