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Code Name Applications Availability Size Tested species Price Grade  
EB06104 Goat Anti-ANILLIN / Scraps (C Terminus) Antibody Pep-ELISA, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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EB06104 1
EB06104 (10µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
EB06104 2
EB06104 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06104 3
EB06104 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ANLN, Scraps, ANILLIN, anillin, actin binding protein (scraps homolog, Drosophila), anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, DKFZp779A055, scra
Official Symbol: ANLN
Accession Number(s): NP_061155.2; NP_001271230.1; NP_001271231.1
Human GeneID(s): 54443
Non-Human GeneID(s): 68743 (mouse)

Immunogen

Peptide with sequence WQPDACYKPIGKP, from the C Terminus of the protein sequence according to NP_061155.2; NP_001271230.1; NP_001271231.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
IHC: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration: 3-10µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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