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Goat Anti-POLL / POLkappa Antibody (EB06206)

Related Products: EBP06206 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06206 Goat Anti-POLL / POLkappa Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06206 1
EB06206 (0.5µg/ml) staining of A549 (A) and (1ug/ul) HepG2 (B) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06206 2
EB06206 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: POLL, BETA-N, POL-KAPPA, polymerase (DNA directed), lambda, polymerase (DNA-directed), lambda, DNA polymerase kappa (DNA polymerase beta-N), RP11-529I10.1, BETAN, FLJ46002, POLKAPPA, DNA polymerase beta-2, DNA polymerase kappa (DNA polymerase beta-N), DNA polymerase lamda2, DNA-directed DNA polymerase lambda, OTTHUMP00000020323, pol beta2, pol lambda
Official Symbol: POLL
Accession Number(s): NP_037406.1
Human GeneID(s): 27343
Non-Human GeneID(s): 56626 (mouse)
Important Comments: It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.

Immunogen

Peptide with sequence C-LGLPYREPAERDW, from the C Terminus of the protein sequence according to NP_037406.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in cell lysates of A549 and approx 60-70kDa in cell lysates of HepG2 (calculated MW of 63.5kDa according to Human NP_037406.1). Recommended concentration: 0.5-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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