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Goat Anti-SM22 Alpha / Transgelin Antibody (EB06281)

Related Products: EBP06281 - Immunizing Peptide, Goat Anti-SM22 Alpha / Transgelin Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB06281 Goat Anti-SM22 Alpha / Transgelin Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc
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EB06281 1
EB06281 (0.03µg/ml) staining of Human Skeletal muscle (A) and (0.003ug/ml) Testes (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06281 2
EB06281 (0.03µg/ml) staining of Mouse Skeletal muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TAGLN, SM22, SMCC, WS3-10, transgelin, SM22-alpha, DKFZp686P11128, TAGLN1, DKFZp686B01212, transgelin variant 2
Official Symbol: TAGLN
Accession Number(s): NP_003177.2
Human GeneID(s): 6876
Non-Human GeneID(s): 21345 (mouse), 25123 (rat)
Important Comments: NP_003177.2 and NP_001001522.1 represent identical protein. The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumour suppressor.

Immunogen

Peptide with sequence C-MTGYGRPRQIIS, from the C Terminus of the protein sequence according to NP_003177.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 24kDa band observed in Human Skeletal Muscle and Testis lysates, and approx 25kDa in Mouse Skeletal Muscle lysates (calculated MW of 22.6kDa according to Human NP_003177.2 and Mouse NP_035656.1). These molecular weights ares routinely observed by other sources. Recommended concentration: 0.003-0.03 µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific References

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Angela Corvino et al.
Antagonizing S1P3 Receptor with Cell-Penetrating Pepducins in Skeletal Muscle Fibrosis
Int J Mol Sci. 2021 Aug; 22(16): 8861.
PMID: 34445567

This antibody (previous batch) has been successfully used in Western blot on Human:
Caterina Bernacchioni, Tommaso Capezzuoli, Valentina Vannuzzi, Francesca Malentacchi, Francesca Castiglione, Francesca Cencetti, Marcello Ceccaroni, Chiara Donati, Paola Bruni, Felice Petraglia
Sphingosine 1-phosphate receptors are dysregulated in endometriosis: possible implication in transforming growth factor β-induced fibrosis
Fertil Steril. 2020 Sep 6;S0015-0282(20)30766-4.
PMID: 32907751

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Bruno G, Cencetti F, Pertici I, Japtok L, Bernacchioni C, Donati C, Bruni P.
CTGF/CCN2 exerts profibrotic action in myoblasts via the up-regulation of sphingosine kinase-1/S1P3 signaling axis: Implications in the action mechanism of TGFβ.
Biochim Biophys Acta. 2015 Feb;1851(2):194-202.
PMID: 25457224

This antibody (previous batch) has been successfully used in Western blot on Human:
Donati C, Marseglia G, Magi A, Serratì S, Cencetti F, Bernacchioni C, Nannetti G, Benelli M, Brunelli S, Torricelli F, Cossu G, Bruni P.
Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6.
PLoS One. 2011;6(5):e20389.
PMID: 21629665

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Cencetti F, Bernacchioni C, Nincheri P, Donati C, Bruni P.
Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis.
Mol Biol Cell. 2010 Mar 15;21(6):1111-24.
PMID: 20089836

This antibody (previous batch) has been successfully used in Western blot on Human:
Nincheri P, Luciani P, Squecco R, Donati C, Bernacchioni C, Borgognoni L, Luciani G, Benvenuti S, Francini F, Bruni P.
Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells.
Cell Mol Life Sci. 2009 May;66(10):1741-54.
PMID: 19337690

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