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Goat Anti-PIK3C2A Antibody (EB06291)

Related Products: EBP06291 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06291 Goat Anti-PIK3C2A Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06291 1
EB06291 (2µg/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06291 2
EB06291 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
EB06291 3
EB06291 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PIK3C2A, CPK, PI3-K-C2A, PI3K-C2alpha, PI3-K-C2(ALPHA), phosphoinositide-3-kinase, class 2, alpha polypeptide, C2-containing phosphatidylinositol kinase, DKFZp686L193, MGC142218
Official Symbol: PIK3C2A
Accession Number(s): NP_002636.2
Human GeneID(s): 5286
Non-Human GeneID(s): 18704 (mouse)

Immunogen

Peptide with sequence C-TVKWYQLTAATYL, from the C Terminus of the protein sequence according to NP_002636.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1µg/ml.
IHC: In paraffin embedded Human Placenta shows cytoplasm staining of cytotrophoblasts and mesenchymal cells. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

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