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Goat Anti-RGS18 Antibody (EB06372)

Code Name Applications Availability Size Tested species Price Grade  
EB06372 Goat Anti-RGS18 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB06372 1
EB06372 (1µg/ml) staining of Human Peripheral Blood Mononucleocytes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06372 2
EB06372 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06372 3
EB06372 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RGS18, RGS13, regulator of G-protein signalling 18, regulator of G-protein signaling 13, RP11-142L4.1, regulator of G-protein signalling 13
Official Symbol: RGS18
Accession Number(s): NP_570138.1
Human GeneID(s): 64407

Immunogen

Peptide with sequence CNEFQDVQSDVAIWL, from the C Terminus of the protein sequence according to NP_570138.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 28kDa band observed in Human Peripheral Blood Mononucleocytes (lymphocytes) lysates (calculated MW of 27.6kDa according to NP_570138.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Prostate and Small Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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