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Goat Anti-TEM8 / Anthrax Toxin Receptor 1 Antibody (EB06472)

Related Products: EBP06472 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06472 Goat Anti-TEM8 / Anthrax Toxin Receptor 1 Antibody Pep-ELISA, IF Contact sales for delivery estimate 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Email: [email protected]
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EB06472 1
EB06472 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: TEM8, anthrax toxin receptor, ATR, FLJ10601, FLJ21776, tumor endothelial marker 8, tumor endothelial marker 8, isoform 3 precursor, ANTXR1, anthrax toxin receptor 1, FLJ11298, 2310008J16Rik, 2810405N18Rik
Official Symbol: ANTXR1
Accession Number(s): NP_115584.1
Human GeneID(s): 84168
Important Comments: This antibody is expected to recognise variant 1 (NP_115584) only.

Immunogen

Peptide with sequence C-RAPPPSRPPPRPSV, from the C Terminus of the protein sequence according to NP_115584.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary testing showed a band at approx 65-70kDa in lysate of cell lines A431 and U2OS after 1-2µg/ml antibody staining (calculated MW of 62.8kDa according to NP_115584.1). This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the membrane and cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog, Cow

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